[1]何 清,李 敏,唐文诚,等.电离辐照损伤对小鼠脾脏组织核糖体信号通路的影响[J].中国药理学通报,2019,(09):1244-1250.[doi:10.3969/j.issn.1001-1978.2019.09.012]
 HE Qing,LI Min,TANG Wen-cheng,et al.Effects of ionizing irradiation on ribosome signaling pathway in spleen tissues of mice[J].Chinese Pharmacological Bulletin,2019,(09):1244-1250.[doi:10.3969/j.issn.1001-1978.2019.09.012]
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电离辐照损伤对小鼠脾脏组织核糖体信号通路的影响()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2019年09期
页码:
1244-1250
栏目:
论著
出版日期:
2019-09-09

文章信息/Info

Title:
Effects of ionizing irradiation on ribosome signaling pathway in spleen tissues of mice
文章编号:
1001-1978(2019)09-1244-07
作者:
何 清1李 敏2唐文诚2易辉燕2王 蕾2王继生12
1.西南医科大学药学院,四川 泸州 646000; 2.绵阳市第三人民医院(四川省精神卫生中心),四川 绵阳 621000
Author(s):
HE Qing1 LI Min2 TANG Wen-cheng2 YI Hui-yan2 WANG Lei2 WANG Ji-sheng12
1.College of Pharmacy, Southwest Medical University, Luzhou Sichuan 646000, China; 2.the Third People's Hospital of Mianyang, Sichuan Mental Health Center, Mianyang Sichuan 621000, China
关键词:
电离辐照 脾脏组织 比较蛋白质组学 ITRAQ 核糖体 KEGG
Keywords:
ionizing radiation spleen tissues comparative proteomics ITRAQ ribosome KEGG
分类号:
R-332; R322.21; R341.31; R641; R852.7; R977.6
DOI:
10.3969/j.issn.1001-1978.2019.09.012
文献标志码:
A
摘要:
目的 从比较蛋白质组学的角度,分析电离辐照损伤组小鼠与正常组小鼠脾脏组织差异蛋白质的表达情况,对差异蛋白所涉及的信号通路进行分析,从而阐明电离辐照对小鼠脾脏组织的损伤机制。方法 采用5 Gy 60Co γ射线照射小鼠,构建小鼠电离辐照损伤模型,提取电离辐照组小鼠和正常组小鼠的脾脏组织,利用ITRAQ联合LC-MS/MS技术,筛选出两组小鼠脾脏组织的差异蛋白质。结果 KEGG Pathway富集分析共鉴定到17条生物信号通路(P<0.05),其中富集在核糖体信号通路的差异蛋白质有37个,其中有36个差异蛋白质表达下调,1个差异蛋白质表达上调。这些通路涉及核糖体蛋白、60S核糖体蛋白、40S核糖体蛋白等的多个亚基蛋白,这些蛋白主要参与蛋白质的翻译及核糖体的结构组成等生物过程。结论 电离辐照导致小鼠脾脏组织核糖体信号通路所涉及的37个差异蛋白中,有36个差异蛋白表达下调,1个差异蛋白表达上调,导致脾脏组织蛋白质合成障碍是电离辐照损伤的重要机制。
Abstract:
Aim To clarify the damage mechanisms of ionizing radiation on mouse spleen tissues and analyse differential protein expression in spleen tissues of mice exposed to ionizing radiation injury and normal mice from the perspective of comparative proteomics, in order to analyse the signaling pathways involved in differential proteins.Methods The mouse ionizing radiation injury model was established by 5 Gy 60Co γ ray, and the mouse spleen tissue was extracted in the ionizing radiation group and normal group, then ITRAQ combined with LC-MS/MS detection technology were used to screen differentially expressed proteins of spleen tissues from ionizing radiation group and normal group.Results A total of 17 biological signaling pathways were identified by KEGG pathway enrichment analysis(P<0.05), in which 37 differential expressed proteins were enriched in the ribosome signaling pathway, including 36 differential expressed proteins down-regulated and one differential expressed protein up-regulated.These pathway involved several multiple subunit proteins, such as ribosome proteins, 60S ribosome proteins and 40S ribosome proteins, which were mainly involved in biological processes, such as translation of proteins, structural composition of ribosome and so on.Conclusions Ionizing radiation causes 37 differential proteins involved in ribosome signaling pathway of mouse spleen tissues, including 36 differential expressed proteins down-regulated and one differential expressed protein up-regulated.The disturbance of protein synthesis in spleen tissues is an important mechanism of ionizing radiation injury.

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备注/Memo

备注/Memo:
收稿日期:2019-04-18,修回日期:2019-05-22
基金项目:四川省应用基础研究计划项目(No 2014JY0058)
作者简介:何 清(1994-),女,硕士生,研究方向:生物药剂学与临床药学,E-mail:1249984895@qq.com;
王继生(1974-),男,博士,主任药师,硕士生导师,研究方向:神经精神药理学及药物蛋白质组,通讯作者,E-mail:24475434@qq.com
更新日期/Last Update: 2019-08-14