[1]郝艳艳,聂春霞,何 盼,等.基于 1H NMR代谢组学技术探讨党参不同炮制品对免疫抑制大鼠的影响[J].中国药理学通报,2019,(09):1266-1273.[doi:10.3969/j.issn.1001-1978.2019.09.016]
 HAO Yan-yan,NIE Chun-xia,HE Pan,et al.Effects of different processed products of Codonopsis pilosula on immunosuppressive rats based on 1H NMR metabolomics[J].Chinese Pharmacological Bulletin,2019,(09):1266-1273.[doi:10.3969/j.issn.1001-1978.2019.09.016]
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基于 1H NMR代谢组学技术探讨党参不同炮制品对免疫抑制大鼠的影响()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2019年09期
页码:
1266-1273
栏目:
论著
出版日期:
2019-09-09

文章信息/Info

Title:
Effects of different processed products of Codonopsis pilosula on immunosuppressive rats based on 1H NMR metabolomics
文章编号:
1001-1978(2019)09-1266-08
作者:
郝艳艳1聂春霞2何 盼1武晓伟1刘 聪1郝旭亮1
1.山西省中医药研究院中药方剂研究所,山西 太原 030045; 2.山西省中医药大学中药学院,山西 太原 030619
Author(s):
HAO Yan-yan1 NIE Chun-xia2 HE Pan1 WU Xiao-wei1 LIU Cong1 HAO Xu-liang1
1.Prescription Dept, Shanxi Institute of Traditional Chinese Medicine, Taiyuan 030045, China; 2.College of Traditional Chinese Medicine, Shanxi University of Traditional Chinese Medicine, Taiyuan 030619, China
关键词:
1H NMR 党参 米炒党参 蜜炙党参 免疫抑制 代谢组学
Keywords:
1H NMR Codonopsis pilosula rice-fried Codonopsis pilosula honey-fried Codonopsis pilosula immunosuppression metabonomics
分类号:
R-332; R282.71; R283; R349.1; R445.2; R446.11; R392.12; R979.1
DOI:
10.3969/j.issn.1001-1978.2019.09.016
文献标志码:
A
摘要:
目的 基于核磁共振(1H NMR)代谢组学技术结合多元统计分析,研究环磷酰胺(CTX)所致免疫抑制大鼠血清中内源性代谢产物的变化规律,筛选党参、米炒党参、蜜炙党参提高免疫力的最佳炮制品,并探讨其作用机制。方法 采用腹腔注射CTX复制免疫抑制模型,给药最后1 d收集大鼠血液,进行免疫细胞、免疫因子检测以及1H NMR代谢组学测试。结果 党参及其炮制品均可提高免疫低下大鼠血清中白细胞、B淋巴细胞数及T细胞CD4+/CD8+值; 党参、蜜炙党参调节IL-2、IgG、sIgA的效果优于米炒党参; 蜜炙党参可明显改善免疫低下大鼠血清中亮氨酸、异亮氨酸、丙氨酸、谷氨酸、谷氨酰胺、萄葡糖、缬氨酸等物质的含量。结论 蜜炙党参改善CTX所致的免疫低下效果最好,主要通过3条代谢通路来调节,分别为缬氨酸、亮氨酸、异亮氨酸代谢,谷氨酸、谷氨酰胺代谢,以及丙氨酸、天冬氨酸、谷氨酸代谢。
Abstract:
Aim Based on 1H NMR metabonomics combined with multivariate statistical analysis, to study the changes of serum endogenous metabolites from immunosuppressive rats induced by cyclophosphamide(CTX), and to screen the best processed products of Codonopsis pilosula, rice-fried Codonopsis pilosula and honey-fried Codonopsis pilosula in improve immunity, and to explore its mechanisms.Methods CTX was administered intraperitoneally(i.p.)to induce the immunosuppressive model.Serum was collected by aorta abdominalis on the last day for detecting immunocyte, immunokine and 1H NMR analysis.Results Codonopsis pilosula and its processed products could increase the numbers of white blood cells and B lymphocytes, as well as the ratio of CD4+ and CD8+ T cells.The regulation effects of honey-fried Codonopsis pilosula and Codonopsis pilosula on IL-2, IgG and sIgA were better than those of rice-fried Codonopsis pilosula.Honey-fried Codonopsis pilosula could significantly improve the levels of leucine, isoleucine, alanine, glutamate, glutamine, glucose and valine etc.Conclusions Honey-fried Codonopsis pilosula could play the best role in immunosuppressive rats through regulating valine, leucine and isoleucine metabolism, glutamate and glutamine metabolism, alanine, aspartate and glutamate metabolism.

参考文献/References:

[1] 中国药典委员会[S].中华人民共和国药典.2015年版一部.北京:中国医学科学出版社,2015:281-2.
[1] Chinese Pharmacopoeia Commission.Pharmacopoeia of the People's Republic of China[S].2015 ed.Part 1.Beijing: China Medical Science Press, 2015: 281-2.
[2] 张晓君,祝晨蔯,胡 黎,等.党参多糖对小鼠免疫和造血功能的影响[J].中药新药与临床药理,2003,14(3):174-6.
[2] Zhang X J, Zhu C C, Hu L, et al.Effects of Codonopsis pilosula polysaccharides on immunity and hematopoietic function in mice[J].Tradit Chin Drug Res Pharmacol, 2003,14(3): 174-6.
[3] 张雅君,梁忠岩,张丽霞.党参粗多糖的组成及其免疫活性研究[J].西北农林科技大学学报(自然科学版),2012,40(7):199-202, 208.
[3] Zhang Y J, Liang Z Y, Zhang L X.Composition and immunological activity of crude polysaccharide from Dang Shen[J].J Nort A&F Univ(Natur Sci),2012,40(7):199-202, 208.
[4] 吴尚英,范圣此,关扎根,琚斌峰.影响党参药材品质的因素分析[J].安徽农业科学,2011,39(30):18528-30.
[4] Wu S Y, Fan S C, Guan Z G, Ju B F.Anaiysis of factors influencing the quality of Dang Shen medicinal materials[J].J Anhui Agric Sci, 2011,39(30):18528-30.
[5] 邹 利,邱炳勋,刘 珂,等.党参米炒前后党参多糖与5-羟甲基糠醛的变化及其对胃肠平滑肌运动的影响[J].中草药,2017,48(1):149-54.
[5] Zhou L, Qiu B X, Liu K, et al.Changes of 5-hydroxymethyl furfural of Codonopsis pilosula polysaccharides before and after stir-frying and its effect on gastrointestinal smooth muscle movement[J].Chin Tradit Herb Drug,2017,48(1):149-54.
[6] 杨中林,刘海臣,林 蔚.党参的炮制研究[J].中药材,1990,23(4):25-6.
[6] Yang Z L, Liu H C, Lin W.Study on processing of Codonopsis pilosula[J].Chin Med Mat,1990,23(4):25-6.
[7] 斯皮热古丽·阿布都卡地,阿里木·帕塔尔,古孜力克孜·阿布都克热木.基于NMR的免疫抑制小鼠模型血清代谢组学研究[J].中国药理学通报,2016,32(6):853-7.
[7] Sipireguli·Abudoukadi, Alimu·Pataer, Guzilikezi·Abudoukeremu.Metabolic analysis of immune suppression mice based on NMR[J].Chin Pharmacol Bull, 2016,32(6):853-7.
[8] 刘成鹏,殷晓芹,李玉琴,等.基于核磁共振的益母草碱抗急性心肌缺血的代谢组学研究[J].中国药理学通报,2017,33(9):1235-42.
[8] Liu C P,Yin X Q,Li Y Q, et al.1H NMR-based metabolomics study of intervention effects of leonurine on acute myocardial ischemia in rats[J].Chin Pharmacol Bull, 2017,33(9):1235-42.
[9] 龚梦鹃,巫圣乾,岳 贺,等.基于1H-NMR护肝片抗大鼠急性肝损伤的代谢组学研究[J].中国药理学通报,2017,33(12):1766-70.
[9] Gong M J,Wu S Q,Yue H,et al.Metabonomic analysis of Hugan Tablets on CCl4-induced acute hepatic injury in rats based on 1H-NMR[J].Chin Pharmacol Bull, 2017,33(12):1766-70.
[10] Lämmermann T, Germain R N.The multiple faces of leukocyte interstitial migration[J].Semin Immunopathol,2014, 36(2): 227-51.
[11] Cho J H, Boyman O, Kim H O, et al.An intense form of homeostatic proliferation of naive CD8+ cells driven by IL-2[J].J Exp Med, 2007, 204(8):1787-801.
[12] 罗治彬.肠道黏膜SIgA免疫系统的研究进展[J].细胞与分子免疫学杂志,1997,13(S2):40-3.
[12] Luo Z B.Progress of sIgA immune system in intestinal mucosa[J].J Cell Mol Immunol,1997,13(S2):40-3.
[13] Les I, Doval E, García-Martínez R.Effects of branched-chain amino acids supplementation in patients with cirrhosis and a previous episode of hepatic encephalopathy: a randomized study[J].Am J Gastroenterol, 2011,106(6):1081-8.
[14] Zhao L, Li Y, Li Z, et al.Effect of the valine-to-lysine ratio on the performance of sows and piglets in a hot, humid environment[J].J Therm Biol, 2019,81:89-97.
[15] 毕晓林,何伟东.谷氨酰胺对病毒性脑炎小鼠免疫功能的影响[J].南京医科大学学报(自然科学版),2007,(9):1029-30.
[15] BI X L, HE W D.Effects of glutamine on immune function in mice with viral encephalitis[J].Acta Acad Med Nanjing(Natur Sci), 2007,(9):1029-30.
[16] Duval D, Demangel C, Munier-Jolain K, et al.Factors controlling cell proliferation and antibody production in mouse hybridoma cells: I.Influence of the amino acid supply[J].Biotechnol Bioeng, 1991, 38(6): 561-70.
[17] Deja S, Porebska I, Kowal A, et al.Metabolomics provide new insights on lung cancer staging and discrimination from chronic obstructive pulmonary disease[J].J Pharm Biomed Anal, 2014,100:369-80.
[18] de Graaff D R, Felz S, Neu T R, et al.Sialic acids in the extracellular polymeric substances of seawater-adapted aerobic granular sludge[J].Water Res,2019,155:343-51.

备注/Memo

备注/Memo:
收稿日期:2019-05-10,修回日期:2019-06-18
基金项目:国家中药标准化项目(No ZYBZ-Y-JIN-34)
作者简介:郝艳艳(1992-),女,硕士生,研究方向:中药药效物质基础及作用机制,E-mail: 360262997@qq.com;
郝旭亮(1975-),男,博士,主任药师,研究方向:中药作用物质基础及作用机制,通讯作者,E-mail: hxliang-01@163.com
更新日期/Last Update: 2019-08-14