[1]徐莉莎,朱家桢,冯黎黎,等.富硒长双歧杆菌水溶性蛋白保护脂多糖诱导的大鼠肠上皮细胞IEC6损伤[J].中国药理学通报,2019,(09):1274-1278.[doi:10.3969/j.issn.1001-1978.2019.09.017]
 XU Li-sha,ZHU Jia-zhen,Feng Li-li,et al.The protective effect of water-soluble proteins from selenium-enriched Bifidobacterium longum on lipopolysaccharide-induced injury in rat IEC6 cells[J].Chinese Pharmacological Bulletin,2019,(09):1274-1278.[doi:10.3969/j.issn.1001-1978.2019.09.017]
点击复制

富硒长双歧杆菌水溶性蛋白保护脂多糖诱导的大鼠肠上皮细胞IEC6损伤()
分享到:

《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2019年09期
页码:
1274-1278
栏目:
论著
出版日期:
2019-09-09

文章信息/Info

Title:
The protective effect of water-soluble proteins from selenium-enriched Bifidobacterium longum on lipopolysaccharide-induced injury in rat IEC6 cells
文章编号:
1001-1978(2019)09-1274-05
作者:
徐莉莎朱家桢冯黎黎吴旭东
南京大学生命科学学院医药生物技术国家重点实验室,江苏 南京 210023
Author(s):
XU Li-sha ZHU Jia-zhen Feng Li-li WU Xu-dong
State Key Lab of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China
关键词:
富硒长双歧杆菌水溶性蛋白 脂多糖 大鼠肠上皮细胞 细胞紧密连接 细胞凋亡 线粒体膜电位
Keywords:
water soluble proteins from selenium-enriched Bifidobacterium longum lipopolysaccharide rat intestinal epithelial cells tight junction cell apoptosis mitochondria membrane potential
分类号:
R-332; R322.45; R378.994; R329.24; R341; R574; R916.3
DOI:
10.3969/j.issn.1001-1978.2019.09.017
文献标志码:
A
摘要:
目的 本研究用脂多糖诱导大鼠肠上皮细胞IEC6损伤模型,考察富硒长双歧杆菌制剂对IEC6的保护作用。方法 实验分5组,分别为阴性对照组(磷酸平衡盐溶液处理)、模型组(脂多糖处理)、富硒长双歧杆菌水溶性蛋白低、中、高3个浓度组(脂多糖处理的同时分别加入10、30、100 mg·L-1的富硒长双歧杆菌水溶性蛋白)。脂多糖处理24 h后,检测IEC6细胞活力、细胞凋亡、线粒体膜电位、细胞紧密连接蛋白1(zonula occludens 1, ZO-1)和闭合素(Occludin)的表达。结果 脂多糖可导致大鼠肠上皮细胞IEC6损伤,表现为细胞活力下降,凋亡增加,线粒体膜电位倒塌,细胞紧密连接蛋白表达减少。富硒长双歧杆菌水溶性蛋白能够抑制脂多糖诱导的IEC6细胞损伤,减少细胞凋亡,增强细胞间紧密连接。结论 富硒长双歧杆菌水溶性蛋白能够保护脂多糖诱导的IEC6细胞损伤。
Abstract:
Aim To investigate the protective effect of selenium-enriched Bifidobacterium longum preparation on rat IEC6 cells via the application of lipopolysaccharide(LPS)-induced IEC6 cell injury model.Methods The experiment was divided into five groups: negative control group treated with phosphoric acid equilibrium salt solution, model group treated with LPS, and low, medium, high concentration group in which IEC6 cells were treated with LPS plus 10, 30, 100 mg·L-1 water-soluble proteins from selenium-enriched Bifidobacterium longum respectively.After treatment with LPS for 24 hours, IEC6 cell viability, apoptosis, mitochondria membrane potential, and the expression of zonula occludens 1(ZO-1)and Occludin were detected.Results LPS induced rat intestinal epithelial cell damage, such as the decrease of cell viability, the increase of cell apoptosis, the collapse of mitochondria membrane potential, and the decrease of cell tight junction protein expression.Water-soluble proteins from selenium-enriched Bifidobacterium longum inhibited LPS-induced IEC6 cell damage, decreased cell apoptosis and increased cell tight junction between cells.Conclusion Water-soluble proteins from selenium-enriched Bifidobacterium longum have protective effect on LPS-induced rat intestinal epithelial cell injury.

参考文献/References:

[1] Suenaert P, Bulteel V, Lemmens L, et al.Anti-tumor necrosis factor treatment restores the gut barrier in Crohn's disease[J].Am J Gastroenterol, 2002,97(8): 2000-4.
[2] Seong M A, Woo J K, Kang J H, et al.Oral administration of fermented wild ginseng ameliorates DSS-induced acute colitis by inhibiting NF-kappaB signaling and protects intestinal epithelial barrier[J].BMB Rep, 2015,48(7): 419-25.
[3] Chen S, Zhu J, Chen G, et al.1,25-Dihydroxyvitamin D3 preserves intestinal epithelial barrier function from TNF-alpha induced injury via suppression of NF-kB p65 mediated MLCK-P-MLC signaling pathway[J].Biochem Biophys Res Commun,2015,460(3): 873-8.
[4] Groschwitz K R, Wu D, Osterfeld H, et al.Chymase-mediated intestinal epithelial permeability is regulated by a protease-activating receptor/matrix metalloproteinase-2-dependent mechanism[J].Am J Physiol Gastrointest Liver Physiol, 2013,304(5): G479-89.
[5] Liu F, Cottrell J J, Furness J B, et al.Selenium and vitamin E together improve intestinal epithelial barrier function and alleviate oxidative stress in heat-stressed pigs[J].Exp Physiol, 2016,101(7): 801-10.
[6] Ibrahim H A, Zhu Y, Wu C, et al.Selenium-enriched probiotics improves murine male fertility compromised by high fat diet[J].Biol Trace Elem Res, 2012,147(1-3): 251-60.
[7] Gan F, Ren F, Chen X, et al.Effects of selenium-enriched probiotics on heat shock protein mRNA levels in piglet under heat stress conditions[J].J Agric Food Chem, 2013,61(10): 2385-91.
[8] Nido S A, Shituleni S A, Mengistu B M, et al.Effects of selenium-enriched probiotics on lipid metabolism, antioxidative status, histopathological lesions, and related gene expression in mice fed a high-fat diet[J].Biol Trace Elem Res, 2016,171(2): 399-409.
[9] 王 亮, 杨 芳, 回斯美, 等.芍药苷抑制肌球蛋白轻链激酶缓解肠上皮细胞屏障功能紊乱[J].中国药理学通报, 2017,33(11): 1541-5.
[9] Wang L, Yang F, Hui S M, et al.Paeoniflorin alleviates TNF-α induced intestinal barrier dysfunction by inhibition of MLCK [J].Chin Pharmacol Bull, 2017,33(11): 1541-5.
[10] Song Y A, Park Y L, Yoon S H, et al.Black tea polyphenol theaflavin suppresses LPS-induced ICAM-1 and VCAM-1 expression via blockage of NF-kappaB and JNK activation in intestinal epithelial cells[J].Inflamm Res, 2011,60(5): 493-500.
[11] Cianciulli A, Calvello R, Cavallo P, et al.Modulation of NF-kappaB activation by resveratrol in LPS treated human intestinal cells results in downregulation of PGE2 production and COX-2 expression[J].Toxicol In Vitro, 2012,26(7): 1122-8.
[12] Ling X, Linglong P, Weixia D, Hong W.Protective effects of bifidobacterium on intestinal barrier function in LPS-induced enterocyte barrier injury of Caco-2 monolayers and in a rat NEC model[J].PLoS One, 2016,11(8): e0161635.
[13] Lei Q, Qiang F, Chao D, et al.Amelioration of hypoxia and LPS-induced intestinal epithelial barrier dysfunction by emodin through the suppression of the NF-kappaB and HIF-1alpha signaling pathways[J].Int J Mol Med, 2014,34(6): 1629-39.
[14] Gan F, Chen X, Liao S F, et al.Selenium-enriched probiotics improve antioxidant status, immune function, and selenoprotein gene expression of piglets raised under high ambient temperature[J].J Agric Food Chem, 2014,62(20): 4502-8.
[15] Turner J R.Intestinal mucosal barrier function in health and disease[J].Nat Rev Immunol, 2009,9(11): 799-809.

相似文献/References:

[1]兰芳,何蓉蓉,姚楠,等.叶黄素对小鼠葡萄膜炎氧化应激状态的改善作用[J].中国药理学通报,2010,(09):1257.
 LAN Fang,HE Rong rong,YAO Nan,et al.comImproving effects of lutein on oxidative stress status in endotoxininduced murine uveitis[J].Chinese Pharmacological Bulletin,2010,(09):1257.
[2]刘卓,金英,刘婉珠,等.知母皂苷对脂多糖引起的大鼠学习记忆障碍和炎症反应的影响[J].中国药理学通报,2010,(10):1362.
 LIU Zhuo,JIN Ying,LIU Wan zhu,et al.Effects of SAaB on LPSinduced learning and memory disorders and inflammatory reaction in rat hippocampus[J].Chinese Pharmacological Bulletin,2010,(09):1362.
[3]刘焕龙,朱忠宁,江 平,等.Apelin/APJ系统在脂多糖诱导大鼠肺微血管内皮细胞损伤中的变化及其作用研究[J].中国药理学通报,2015,(08):1152.[doi:10.3969/j.issn.1001-1978.2015.08.025]
 LIU Huan-long,ZHU Zhong-ning,JIANG Ping,et al.The changes and effects of Apelin/APJ system in LPS-induced injury of rat pulmonary microvascular endothelial cells[J].Chinese Pharmacological Bulletin,2015,(09):1152.[doi:10.3969/j.issn.1001-1978.2015.08.025]
[4]张晓晖,曾繁典,孙智达,等.原花青素B2对LPS诱导的心肌细胞凋亡的保护作用[J].中国药理学通报,2015,(11):1510.[doi:10.3969/j.issn.1001-1978.2015.11.008]
 ZHANG Xiao-hui,ZENG Fan-dian,SUN Zhi-da,et al.Procyanidin B2 protects LPS-induced myocardial cell apoptosis[J].Chinese Pharmacological Bulletin,2015,(09):1510.[doi:10.3969/j.issn.1001-1978.2015.11.008]
[5]刘丹丹,曹 纲,张 琦,等.三叶青黄酮经p38MAPK和NF-κB途径抑制老年小鼠急性肺损伤[J].中国药理学通报,2015,(12):1725.[doi:10.3969/j.issn.1001.1978.2015.12.019]
 LIU Dan-dan,CAO Gang,ZHANG Qi,et al."Inhibition effect of flavonoids from Radix tetrastigmae on acute lung injury of aged mice through p38MAPK and NF-κB pathway Inhibition effect of flavonoids from Radix tetrastigmae on acute lung injury[J].Chinese Pharmacological Bulletin,2015,(09):1725.[doi:10.3969/j.issn.1001.1978.2015.12.019]
[6]李德川,鲍秀琦,张德武,等.去氢丹参新酮对神经炎症的抑制作用及机制研究[J].中国药理学通报,2016,(02):177.[doi:10.3969/j.issn.1001-1978.2016.02.007]
 LI De-chuan,BAO Xiu-qi,ZHANG De-wu,et al.The anti-neuroinflammatory effects of dehydromiltirone and related mechanisms[J].Chinese Pharmacological Bulletin,2016,(09):177.[doi:10.3969/j.issn.1001-1978.2016.02.007]
[7]花慧莲,黄 超.热休克蛋白70激动剂SW02对脂多糖诱导的巨噬细胞iNOS表达的调控及机制[J].中国药理学通报,2016,(02):279.[doi:10.3969/j.issn.1001-1978.2016.02.025]
 HUA Hui-lian,HUANG Chao.Effects of heat shock protein 70 activator SW02 on lipopolysaccharide-induced expression of inducible nitric oxide synthase in macrophages[J].Chinese Pharmacological Bulletin,2016,(09):279.[doi:10.3969/j.issn.1001-1978.2016.02.025]
[8]袁 琴,袁 丁,周志勇,等.竹节参齐墩果烷皂苷对RAW264.7巨噬细胞SIRT1活性影响及抗炎作用研究[J].中国药理学通报,2016,(03):349.[doi:10.3969/j.issn.1001-1978.2016.03.011]
 YUAN Qin,YUAN Ding,ZHOU Zhi-yong,et al.Anti-inflammatory effect of Chikusetsu oleanane saponin on RAW264.7 cell through regulating SIRT1 activity[J].Chinese Pharmacological Bulletin,2016,(09):349.[doi:10.3969/j.issn.1001-1978.2016.03.011]
[9]杨 敏,陆 雪,黄江菊,等.苯环喹溴铵对脂多糖诱导的人鼻黏膜上皮细胞表达MUC5AC的影响[J].中国药理学通报,2016,(06):783.[doi:10.3969/j.issn.1001-1978.2016.06.010]
 YANG Min,LU Xue,HUANG Jiang-ju,et al.Effects of bencycloquidium bromide on the expression of MUC5AC induced by lipopolysaccharide in cultured human nasal epithelial cells[J].Chinese Pharmacological Bulletin,2016,(09):783.[doi:10.3969/j.issn.1001-1978.2016.06.010]
[10]赵 爽,叶 强,李 涛,等.炎症应激通过激活mTOR通路诱导THP-1巨噬细胞泡沫化的实验研究[J].中国药理学通报,2016,(08):1105.[doi:10.3969/j.issn.1001-1978.2016.08.015]
 ZHAO Shuang,YE Qiang,LI Tao,et al.Experiment of THP-1 macrophage foam-cell formation through mTOR signal pathway activation induced by inflammatory stress[J].Chinese Pharmacological Bulletin,2016,(09):1105.[doi:10.3969/j.issn.1001-1978.2016.08.015]

备注/Memo

备注/Memo:
收稿日期:2019-04-10,修回日期:2019-06-12
基金项目:江苏省自然科学基金资助项目(No BK20161399)
作者简介:徐莉莎(1994-),女,硕士生,研究方向:免疫药理学,E-mail:849935134@qq.com
吴旭东(1979-),女,博士,副教授,研究方向:免疫药理学,通讯作者,Tel:025-89681312,E-mail: xudongwu@nju.edu.cn
更新日期/Last Update: 2019-08-14