[1]孙婧文,朱嵩岳,尹 娜,等.体外受精-胚胎移植术对子代肠系膜血管功能的影响[J].中国药理学通报,2019,(09):1279-1283.[doi:10.3969/j.issn.1001-1978.2019.09.018]
 SUN Jing-wen,ZHU Song-yue,YIN Na,et al.Effect of IVF-ET on mesenteric vascular function in offspring[J].Chinese Pharmacological Bulletin,2019,(09):1279-1283.[doi:10.3969/j.issn.1001-1978.2019.09.018]
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体外受精-胚胎移植术对子代肠系膜血管功能的影响()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2019年09期
页码:
1279-1283
栏目:
论著
出版日期:
2019-09-09

文章信息/Info

Title:
Effect of IVF-ET on mesenteric vascular function in offspring
文章编号:
1001-1978(2019)09-1279-05
作者:
孙婧文朱嵩岳尹 娜伯 乐吴晨婷茅彩萍
苏州大学附属第一医院生殖医学中心,江苏 苏州 215006
Author(s):
SUN Jing-wen ZHU Song-yue YIN Na BO Le WU Chen-ting MAO Cai-ping
Reproductive Medicine Center, the First Affiliated Hospital of Soochow University, Suzhou Jiangsu 215006, China
关键词:
不孕症 体外受精-胚胎移植 子代 肠系膜血管功能 细胞因子 Ca2+信号通路及其调控机制
Keywords:
infertility in vitro fertilization-embryo transfer offspring mesenteric vascular function cytokines calcium signal pathway and its regulation mechanism
分类号:
R-332; R322.12; R321-33; R321.3; R332.32; R348.1; R711.6
DOI:
10.3969/j.issn.1001-1978.2019.09.018
文献标志码:
A
摘要:
目的 研究体外受精-胚胎移植术(in vitro fertilization & embryo transfer,IVF-ET)对子代肠系膜血管收缩功能的影响及其调控机制。方法 建立IVF-ET子代小鼠(出生20周后)模型,与自然妊娠子代进行比较,研究其对血管收缩功能的影响。测量子代小鼠体质量和BMI,通过ELISA及比色法检测心血管相关细胞因子水平。鉴于Ca2+信号通路在调节血管收缩功能中的重要作用,本实验选择了4个代表性基因(Calm2、Itpr2、RyR2、RyR3)进行研究,并通过qPCR检测Calm2、Itpr2、RyR2、RyR3的mRNA表达水平。通过Western blot检测Calm2和Itpr1蛋白表达水平。结果 IVF-ET子代出生20周后有体质量增加、BMI异常的现象,血清NO和AngⅡ浓度明显高于对照组(P<0.05)。实验组的Calm2、Itpr1 mRNA和蛋白表达水平均明显高于对照组(P<0.01),实验组RyR3 mRNA表达水平明显高于对照组(P<0.01),而RyR2 mRNA表达水平明显低于对照组(P<0.01)。结论 与IVF-ET子代血管收缩功能相关的血清学指标及调控基因表达水平的改变,可能与IVF-ET子代患心血管疾病的风险增加密切相关。
Abstract:
Aim To study the effect of in vitro fertilization-embryo transfer(IVF-ET)on the contractile function of mesenteric vessels in offspring mouse model and its regulatory mechanism.Methods Offspring mouse model of IVF-ET(20 weeks after birth)was built to compare with natural born descendant in vascular regulation.The bodyweight and BMI of mice were measured.Serum levels of cardiovascular related cytokines were tested by ELISA and colorimetric method.Samples of mesenteric vascular were from IVF-ET mice and normal controls.Given the vital function in regulating vascular contraction which Ca2+ signaling pathway exhibited, four representative genes(Calm2, Itpr1, RyR2, RyR3)were selectedfor study.And the mRNA expression levels of Calm2, Itpr2, RyR2, RyR3 were tested by qPCR.The protein expression levels of Calm2 and Itpr1 were tested by Western blot.Results Mice born under IVF-ET showed increased bodyweight and abnormal BMI after 20th week, and the serum levels of NO and Ang II were significantly higher than those of control(P<0.05).The expression levels of Calm2, Itpr1 were up-regulated both in molecular and protein levels(P<0.01), RyR3 was up-regulated in molecular level(P<0.01), while RyR2 was down-regulated in molecular level(P<0.01).Conclusions The changes of serological markers and regulatory gene expression level related to vasoconstriction function may be closely related to the increased risk of cardiovascular diseases in IVF-ET offspring.

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备注/Memo

备注/Memo:
收稿日期:2019-04-15,修回日期:2019-05-18
基金项目:国家自然科学基金资助项目(No 81671535); 江苏省社会发展项目(No BE2015642); 江苏省妇幼健康“生殖医学”重点学科项目(No FXΚ201749); 江苏省“科教强卫”医学重点人才项目(No ZDRCA2016044)
作者简介:孙婧文(1993-),女,硕士生,研究方向:生殖医学,E-mail: jwsun417@stu.suda.edu.cn;
茅彩萍(1968-),女,博士,教授,博士生导师,研究方向:生殖医学、围产药理学,通讯作者,E-mail: maocaiping@suda.edu.cn
更新日期/Last Update: 2019-08-14