[1]卢 倩,孙敏哲,杜雯雯,等.复方蜚蠊提取物PB对异体抗原联合醋酸诱导大鼠慢性溃疡性结肠炎的作用机制[J].中国药理学通报,2019,(09):1296-1301.[doi:10.3969/j.issn.1001-1978.2019.09.021]
 LU Qian,SUN Min-zhe,DU Wen-wen,et al.Study on mechanism of action of Blattidae compound PB against chronic ulcerative colitis induced by alloantigen and acetic acid in rats[J].Chinese Pharmacological Bulletin,2019,(09):1296-1301.[doi:10.3969/j.issn.1001-1978.2019.09.021]
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复方蜚蠊提取物PB对异体抗原联合醋酸诱导大鼠慢性溃疡性结肠炎的作用机制()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2019年09期
页码:
1296-1301
栏目:
复方药物药理学
出版日期:
2019-09-09

文章信息/Info

Title:
Study on mechanism of action of Blattidae compound PB against chronic ulcerative colitis induced by alloantigen and acetic acid in rats
文章编号:
1001-1978(2019)09-1296-06
作者:
卢 倩1孙敏哲1杜雯雯1刘 衡1 沈咏梅2吴桃清2赵 昱12巫秀美1
1.大理大学云南省昆虫生物医药研发重点实验室,药用特种昆虫开发国家地方联合工程研究中心,中国西南药用昆虫及蛛形类资源开发利用协同创新中心,云南 大理 671000; 2.药用美洲大蠊四川省重点实验室,四川 成都 610000
Author(s):
LU Qian1 SUN Min-zhe1 DU Wen-wen1 LIU Heng12 SHEN Yong-mei2 WU Tao-qing2 ZHAO Yu12WU Xiu-mei1
1.Dali University Yunnan Provincial Key Lab of Entomological Biopharmaceutical R&D, National-Local Joint Engineering Research Center of Entomoceutics, Yunnan Provincial 2011 Collaborative Innovation Center for Entomoceutics, Dali, Yunnan 671000, China; 2.Sichuan Key Laboratory of Medical American Cockroach,Chengdu 610000, China
关键词:
溃疡性结肠炎 复方 蜚蠊 异体抗原 柳氮磺胺吡啶 作用机制
Keywords:
ulcerative colitics compound Blattidae alloantigen sulfasalazine mechanism of action
分类号:
R-332; R289.5; R384.9; R392.11; R392.12; R574.62
DOI:
10.3969/j.issn.1001-1978.2019.09.021
文献标志码:
A
摘要:
目的 研究复方蜚蠊提取物PB对慢性溃疡性结肠炎(UC)大鼠的作用,并初步探讨其作用机制。方法 采用异体抗原联合醋酸建立慢性UC模型,正常组及模型组灌肠给予氯化钠溶液,其余各组分别给予柳氮磺胺吡啶(SASP)、肠炎宁和PB低、中、高剂量药物。评价疾病活动指数(DAI)和病理组织学评分(HS); 测定脏器指数和结肠黏膜指数(CMDI); ELISA法检测血清中IL-4、IL-8、IL-17、INF-γ、CRP、IgG,以及结肠黏膜中TNF-α、MPO、EGF的含量。结果 与正常组比较,模型组IL-8、IL-17、INF-γ、CRP、IgG、TNF-α、MPO含量均明显升高,IL-4、EGF含量明显降低(P<0.01,P<0.05); PB各剂量组DAI、CMDI、HS、IL-8、IL-17、INF-γ、CRP、IgG、TNF-α、MPO含量均明显降低,IL-4含量明显升高(P<0.01,P<0.05)。结论 复方蜚蠊提取物PB灌肠给药后能够有效缓解异体抗原联合醋酸诱导的大鼠慢性UC,其作用机制可能与促进Th2细胞亚群活化,使倾向于Th1的Th1/Th2平衡向Th2移动,发挥细胞免疫和体液免疫调节作用,达到免疫平衡稳态有关。
Abstract:
Aim To study the effect of Blattidae compound PB on chronic UC induced by alloantigen combined with acetic acid in rats, and to preliminarily explore its mechanism.Methods Chronic UC model was established using alloantigen combined with acetic acid.Normal group and model group were given sodium chloride solution by enema, while the other groups were given respectively to the SASP, the Changyanning and PB low, medium and high doses.The experiment was determined by evaluating DAI, CMDI, HS and the visceral index.Serum IL-4, IL-8, IL-17, CRP, IgG and colonic mucosa TNF- MPO EGF were detected by ELISA.Results Compared with normal group, the levels of IL-8, IL-17, INF-γ, CRP, IgG, TNF-α, MPO in model group significantly increased, while the levels of IL-4, EGF decreased significantly(P<0.01, P<0.05).But the levels of DAI,CMDI, HS, IL-8, IL-17, INF-γ, CRP, IgG, TNF-α, MPO in each dose of PB groups were significantly lower than those of model group, and the levels of IL-4, EGF were significantly higher than those of model group(P<0.01, P<0.05).Conclusions Blattidae compound PB can effectively relieve chronic ulcerative colitis induced by alloantigen combined with acetic acid in rats, and its mechanism may be related to promoting Th2 cell activation, changing Th1-prone Th1/Th2 balance to Th2, which regulated cell immunity and humoral immunity to achieve the steady state immune balance.

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备注/Memo

备注/Memo:
收稿日期:2019-05-18,修回日期:2019-06-10
基金项目:国家自然科学基金资助项目(No 81660605,81860742,81860765); 云南省教育厅科学研究基金产业化培育项目(No 2016CYH15)
作者简介:卢 倩(1994-),女,硕士生,专业方向:中药抗炎药理学,E-mail: luqian940507@163.com;
刘 衡(1989-)男,硕士,研究实习员,研究方向: 中药抗炎与免疫药理学, 通讯作者,E-mail:lheng125@163.com
巫秀美(1968-),女,博士,讲师,研究方向:医学统计学,通讯作者,E-mail:wxm6865@163.com
更新日期/Last Update: 2019-08-14