[1]喻 斌,阮 鸣,许 立,等.川芎、冰片配伍对脑缺血大鼠海马和下丘脑神经元的保护作用[J].中国药理学通报,2019,(09):1302-1308.[doi:10.3969/j.issn.1001-1978.2019.09.022]
 YU Bin,RUAN Ming,XU Li,et al.Synergic effect of Chuanxiong Rhizoma and borneol on protecting hippocampus and hypothalamus of rats from global cerebral ischemia reperfusion injury[J].Chinese Pharmacological Bulletin,2019,(09):1302-1308.[doi:10.3969/j.issn.1001-1978.2019.09.022]
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川芎、冰片配伍对脑缺血大鼠海马和下丘脑神经元的保护作用()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2019年09期
页码:
1302-1308
栏目:
复方药物药理学
出版日期:
2019-09-09

文章信息/Info

Title:
Synergic effect of Chuanxiong Rhizoma and borneol on protecting hippocampus and hypothalamus of rats from global cerebral ischemia reperfusion injury
文章编号:
1001-1978(2019)09-1302-07
作者:
喻 斌1阮 鸣2许 立1刘圣金1许惠琴1沈祥春3
1.南京中医药大学部省共建中药药理实验室,江苏省中药药效与安全性评价重点实验室,江苏 南京 210023; 2.南京晓庄学院食品科学学院,江苏 南京 211117; 3.贵州医科大学天然药物资源优效利用重点实验室,贵州 贵阳 550025
Author(s):
YU Bin1 RUAN Ming2 XU Li1 LIU Sheng-jin1 XU Hui-qin1 SHENG Xiang-chun3
1.Province and Ministry Cooperation Lab of Pharmacology of Chinese Medicine/Jiangsu Key Lab for Pharmacology and Safety Evaluation of Chinese Materia Medica,Nanjing University of Chinese Medicine,Nanjing 210023, China; 2.School of Food Technology, Nanjing Xiaozhuang University, Nanjing 211117, China; 3.the Key Lab of Optimal Utilization of Natural Medicinal Resources, Guizhou Medical University, Guiyang 550025, China
关键词:
川芎 冰片 配伍 全脑缺血/再灌注 兴奋毒 钙超载
Keywords:
Chuanxiong Rhizoma borneol combining therapy GCIR excitotoxicity calcium overload
分类号:
R-332; R289.1; R322.81; R329.25; R348.1; R743.31; R977.4
DOI:
10.3969/j.issn.1001-1978.2019.09.022
文献标志码:
A
摘要:
目的 研究川芎、冰片配伍对全脑缺血大鼠海马和下丘脑神经元的保护机制。方法 将全脑缺血大鼠分为假手术组、模型组、川芎组(1.0 g·kg-1)、冰片组(0.16 g·kg-1)、川芎+冰片组。利用GC-MS方法检测海马/下丘脑的透析液中谷氨酸(glutamate,Glu)、甘氨酸(glycine,Gly)和γ-氨基丁酸(γ-aminobutyric acid,GABA)含量; 激光共聚焦技术检测脑片中钙离子含量; TUNEL和电镜技术检测这两个脑区凋亡百分率和超微结构。结果 川芎和冰片单用均可减少钙超载,缓解超微结构的改变,联合应用效果更佳。另外,川芎单用可减少两脑区的神经元凋亡指数、升高GABA。冰片仅降低两脑区的Glu,而它们的配伍还可进一步升高下丘脑Gly含量,也表现较好的协同关系。结论 川芎和冰片配伍在缓解神经元兴奋毒、钙超载和超微结构异常方面具有较好的协同效应,但抗凋亡方面未见明显协同。
Abstract:
Aim To research the synergic effect of Chuanxiong Rhizoma(CR)and borneol(BO)on protecting hippocampus and hypothalamus of the rats from global cerebral ischemia reperfusion(GCIR)injury.Methods GCIR rats were divided into five groups, i.e.sham, model, CR(1.0 g·kg-1), BO(0.16 g·kg-1)and CR+BO groups.The contents of Glu, Gly and GABA in brain microdialysis solution were detected by GC-MS.[Ca2+]i of the two regions were measured using a confocal microscope.The apoptotic rate and ultrastructure were obtained by TUNEL stain and transmission electron microscopy, respectively.Results The monotherapy of CR or BO attenuated Ca2+ overload and improved ultrastructure in both areas.Their synergy showed a better effect.CR alone reduced the apoptotic rate, increased the levels of GABA in both brain areas, while BO only decreased that of Glu.The combination even enhanced the content of Gly in hypothalamus.Conclusions Obvious synergic effects between CR and BO exist in attenuating neurocellular excitotoxicity and calcium overload, while the anti-apoptotic effect might be excluded.

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备注/Memo

备注/Memo:
收稿日期:2019-04-13,修回日期:2019-05-18
基金项目:国家自然科学基金资助项目(No 81573713); 江苏省自然科学基金资助项目(No BK20151564); 江苏省青蓝工程(2017); 贵州省科技厅项目(黔科合[2016]支撑2826); 贵州省高等学校科技创新团队(No 2014-31); 贵州省科学技术创新团队(No 2015-4025); 高层次创新人才(No 2015-4029)
作者简介:喻 斌(1978-),男,博士,副教授,研究方向:中药药理学,E-mail: nzyyubin@163.com;
阮 鸣(1979-),女,博士,讲师,研究方向:分析化学,并列第一作者,E-mail: 1169195814@qq.com;
沈祥春(1973-),男,博士,教授,研究方向:中药药理学,通讯作者,E-mail:shenxiangchun@126.com
更新日期/Last Update: 2019-08-14