[1]张 雯,宋俊科,朱晓瑜,等.表没食子儿茶素促进Nrf2的核转位减轻Aβ25-35对SH-SY5Y细胞的损伤[J].中国药理学通报,2019,(10):1393-1398.[doi:10.3969/j.issn.1001-1978.2019.10.013]
 ZHANG Wen,SONG Jun-ke,ZHU Xiao-yu,et al.Epigallocatechin promotes nuclear translocation of Nrf2 to reduce damage of Aβ25-35 in SH-SY5Y cells[J].Chinese Pharmacological Bulletin,2019,(10):1393-1398.[doi:10.3969/j.issn.1001-1978.2019.10.013]
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表没食子儿茶素促进Nrf2的核转位减轻Aβ25-35对SH-SY5Y细胞的损伤()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2019年10期
页码:
1393-1398
栏目:
论著
出版日期:
2019-09-15

文章信息/Info

Title:
Epigallocatechin promotes nuclear translocation of Nrf2 to reduce damage of Aβ25-35 in SH-SY5Y cells
文章编号:
1001-1978(2019)10-1393-06
作者:
张 雯1宋俊科1朱晓瑜2杨海光1王海港1许启泰2杜冠华1
1.中国医学科学院北京协和医学院药物研究所,天然药物活性物质与功能国家重点实验室, 药物靶点研究与新药筛选北京市重点实验室,北京 100050; 2.海南绿槟榔科技发展有限公司,海南 定安 571200
Author(s):
ZHANG Wen1 SONG Jun-ke1 ZHU Xiao-yu2Yang Hai-guang1 WANG Hai-gang1 XU Qi-tai2 DU Guan-hua1
1.State Key Lab of Bioactive Substances and Functions of Natural Medicines, Beijing Key Lab of Drug Target and Screening Research, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China; 2.Hainan Green Areca Technology Development Co.LTD, Dingan Hainan 571200, China
关键词:
β淀粉样蛋白 人神经母细胞瘤细胞 表没食子儿茶素 核因子E2相关因子2 氧化应激 神经保护
Keywords:
25-35 SH-SY5Y epigallocatechin Nrf2 oxidative stress neuroprotection
分类号:
R282.71; R322.8; R329.25; R341; R349.1; R745.7
DOI:
10.3969/j.issn.1001-1978.2019.10.013
文献标志码:
A
摘要:
目的 研究表没食子儿茶素(epigallocatechin,EGC)对Aβ25-35损伤SH-SY5Y细胞的保护作用及机制。方法 采用Aβ25-35损伤SH-SY5Y细胞,MTT和LDH法,考察EGC对SH-SY5Y细胞活力的影响。应用DCFH-DA荧光探针检测细胞内ROS水平,通过测定细胞内SOD、GSH-Px和MDA活性,考察细胞氧化应激水平。Western blot测定细胞中Nrf2、NQO1、HO-1、Prdx6和Trx1蛋白含量。结果 Aβ25-35损伤SH-SY5Y细胞,导致细胞存活率明显降低。5、10、20 μmol·L-1 EGC能够减轻Aβ25-35诱导的SH-SY5Y细胞损伤及LDH释放。而且EGC能够减少Aβ25-35诱导的细胞内ROS水平的增加,减轻氧化应激损伤。同时,EGC能够明显增强Aβ25-35损伤后Nrf2、NQO1、HO-1、Prdx6和Trx1的表达。结论 EGC能够抑制Aβ25-35诱导的SH-SY5Y细胞损伤,通过促进Nrf2核转位,提高抗氧化水平,降低Aβ25-35神经毒性。
Abstract:
Aim To investigate the protective effect of epigallocatechin(EGC)on SH-SY5Y cells induced by Aβ25-35 injury.Methods MTT and LDH assay were employed to investigate the effects of EGC on viability of SH-SY5Y cells.DCFH-DA fluorescent probe was used to detect the intracellular ROS levels, and the activity of SOD, GSH-Px and MDA in cells was measured to investigate the level of oxidative stress in cells.Western blot analysis was used to determine Nrf2, NQO1, HO-1, Prdx6 and Trx1 levels in cells.Results SH-SY5Y cells were injured by Aβ25-35 treatment, and the cell viability was significantly reduced.5, 10 and 20 μmol·L-1 of EGC alleviated Aβ25-35 induced SH-SY5Y cell injury and reduced LDH release.In addition, EGC could reduce the intracellular ROS level induced by Aβ25-35 and inhibit oxidative stress.At the same time, EGC could significantly enhance the expression of Nrf2, NQO1, HO-1, Prdx6 and Trx1 after Aβ25-35 injury.Conclusions EGC could inhibit Aβ25-35-induced SH-SY5Y cell injury, increase the cells antioxidant levels and reduce the neurotoxicity of Aβ25-35 by promoting nuclear translocation of Nrf2.

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备注/Memo

备注/Memo:
收稿日期:2019-07-18,修回日期:2019-08-22 基金项目:海南省重大科技计划资助项目(No ZDKJ2016003); 中国医学科学院医学与健康科技创新工程资助项目(No 2017-I2M-1-010) 作者简介:张 雯(1990-),女,博士,助理研究员,研究方向:神经药理学与新药发现,E-mail:zhangwen9010@imm.ac.cn; 许启泰(1955-),男,本科, 教授,研究方向:天然药物药理学,通讯作者,E-mail:xuqitai@vip.sina.com; 杜冠华(1956-),男,博士,研究员,博士生导师,研究方向:神经药理学、心脑血管药理学与新药发现,通讯作者,E-mail:dugh@imm.ac.cn
更新日期/Last Update: 2019-09-15