[1]胡兴华,张峻槐,黄礼义,等.姜黄素-PLGA纳米粒的制备及诱导胶质瘤细胞凋亡的实验研究[J].中国药理学通报,2019,(10):1399-1405.[doi:10.3969/j.issn.1001-1978.2019.10.014]
 HU Xing-hua,ZHANG Jun-huai,HUANG Li-yi,et al.Study on preparation of curcumin-PLGA nanoparticles and their effect on apoptosis in glioma cells[J].Chinese Pharmacological Bulletin,2019,(10):1399-1405.[doi:10.3969/j.issn.1001-1978.2019.10.014]
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姜黄素-PLGA纳米粒的制备及诱导胶质瘤细胞凋亡的实验研究()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2019年10期
页码:
1399-1405
栏目:
论著
出版日期:
2019-09-15

文章信息/Info

Title:
Study on preparation of curcumin-PLGA nanoparticles and their effect on apoptosis in glioma cells
文章编号:
1001-1978(2019)10-1399-07
作者:
胡兴华1张峻槐1黄礼义2徐忠烨1
重庆医科大学附属第二医院1.神经外科、2.康复医学科,重庆 400010
Author(s):
HU Xing-hua1 ZHANG Jun-huai1 HUANG Li-yi2 XU Zhong-ye1
1.Dept of Neurosurgery; 2.Dept of Rehabilitation, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China
关键词:
姜黄素 胶质瘤 凋亡 PLGA C6细胞 U251细胞
Keywords:
curcumin glioma apoptosis PLGA C6 cells U251 cells
分类号:
R-332; R282.71; R318.08; R329.25; R730.264; R916.4
DOI:
10.3969/j.issn.1001-1978.2019.10.014
文献标志码:
A
摘要:
目的 制备姜黄素-PLGA纳米粒(Cur-PLGA-NPs),评价其对胶质瘤细胞的影响。方法 C6、U251细胞传代培养,随机分为对照组、姜黄素组和姜黄素-PLGA组。倒置荧光显微镜、透射电镜观察Cur-PLGA-NPs的形态; 马尔文粒径电位仪检测Cur-PLGA-NPs的粒径; 酶标仪检测Cur-PLGA-NPs的包封率和载药率; 荧光显微镜观察C6、U251细胞对Cur-PLGA-NPs的摄取; CCK-8检测Cur-PLGA-NPs对C6、U251细胞活性的影响; 流式细胞术检测Cur-PLGA-NPs对凋亡的影响; Hoechst染色检测凋亡。结果 Cur-PLGA-NPs的平均粒径为(284.6±9.0)nm,呈球形。其包封率为(70.712±2.615)%,载药率为(2.828±0.105)%。与对照组、姜黄素组相比,C6、U251细胞对Cur-PLGA-NPs的摄取均明显增加(P<0.05); 与对照组、姜黄素组相比,姜黄素-PLGA组的C6、U251细胞活性均明显降低(P<0.05)。与对照组、姜黄素组相比,Cur-PLGA-NPs能更明显引起C6、U251细胞凋亡(P<0.05)。结论 与姜黄素相比,Cur-PLGA-NPs能增强肿瘤细胞对药物的摄取,促进肿瘤细胞凋亡。
Abstract:
Aim To prepare curcumin-PLGA nanoparticles[curcumin poly(lactic-co-glycolic acid)nanoparticles, Cur-PLGA-NPs] and evaluate their effects on glioma cells in vitro.Methods C6 cells and U251 cells were subcultured and randomly divided into control group, curcumin group and curcumin-PLGA group.The morphology of Cur-PLGA-NPs was observed by inverted fluorescence microscope and transmission electron microscopy.The particle size of Cur-PLGA-NPs was detected by Malvern particle size potentiometer.The encapsulation efficiency and drug loading rate of Cur-PLGA-NPs were detected by a multi-function microplate reader.The uptake of Cur-PLGA-NPs by C6 cells and U251 cells was observed by fluorescence microscopy.The effect of Cur-PLGA-NPs on the viability of C6 cells and U251 cells was detected by CCK-8.The effect of Cur-PLGA-NPs on apoptosis was assessed by flow cytometry.Apoptosis was detected by Hoechst staining.Results The Cur-PLGA-NPs had an average particle size of(284.6±9.0)nm and were spherical.The encapsulation efficiency was(70.712±2.615)%, and the drug loading rate was(2.828±0.105)%.Compared with control group and curcumin group, C6 cells and U251 cells significantly increased the uptake of Cur-PLGA-NPs(P<0.05).Compared with control group and curcumin group, the cell viability of C6 cells and U251 cells in curcumin-PLGA group significantly decreased(P<0.05).Compared with control group and curcumin group, the Cur-PLGA-NPs induced apoptosis significantly in C6 cells and U251 cells(P<0.05).Conclusion Compared with curcumin, Cur-PLGA-NPs can enhance the uptake of drugs by tumor cells, promoting tumor cell apoptosis.

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备注/Memo

备注/Memo:
收稿日期:2019-05-14,修回日期:2019-07-24 基金项目:国家自然科学基金青年基金资助项目(No 81471676); 重庆市科委前沿与应用基础研究(一般)项目(No cstc2014jcyjA10050); 重庆市卫生局中医药课题(No ZY20132103); 重庆市卫生局课题(No 2012-2-065) 作者简介:胡兴华(1991-),男,硕士生,研究方向:胶质瘤的治疗,E-mail:huxinghua123@126.com; 徐忠烨(1977-),男,博士,副教授,研究方向:胶质瘤的治疗,通讯作者,E-mail:xuzhongye1977@163.com
更新日期/Last Update: 2019-09-15