[1]徐 珊,周 游,刘钰妮,等.力达霉素对人宫颈癌Hela细胞周期、凋亡、自噬和上皮细胞间质转化能力的影响[J].中国药理学通报,2019,(10):1406-1415.[doi:10.3969/j.issn.1001-1978.2019.10.015]
 XU Shan,ZHOU You,LIU Yu-ni,et al.Effects of Lidamycin on cell cycle, apoptosis, autophagy and epithelial mesenchymal transformation of human cervical carcinoma Hela cells[J].Chinese Pharmacological Bulletin,2019,(10):1406-1415.[doi:10.3969/j.issn.1001-1978.2019.10.015]
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力达霉素对人宫颈癌Hela细胞周期、凋亡、自噬和上皮细胞间质转化能力的影响()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2019年10期
页码:
1406-1415
栏目:
论著
出版日期:
2019-09-15

文章信息/Info

Title:
Effects of Lidamycin on cell cycle, apoptosis, autophagy and epithelial mesenchymal transformation of human cervical carcinoma Hela cells
文章编号:
1001-1978(2019)10-1406-10
作者:
徐 珊12周 游1刘钰妮1王世恩1杨建林1王艳林1曹春雨1
1.三峡大学医学院,肿瘤微环境与免疫治疗湖北省重点实验室,湖北 宜昌 443002; 2.宜昌市疾病预防控制中心,健康管理服务中心,湖北 宜昌 443000
Author(s):
XU Shan12 ZHOU You1 LIU Yu-ni1WANG Shi-en1 YANG Jian-lin1 WANG Yan-lin1 CAO Chun-yu1
1.Medical College of China Three Gorges University, Hubei Key Lab of Tumor Microenvironment and Immunotherapy, Yichang Hubei 443002, China; 2.Yichang Center for Disease Control and Prevention, the Health Management Service Center, Yichang Hubei 443000, China
关键词:
力达霉素 宫颈癌 Hela细胞 细胞凋亡 细胞自噬 上皮细胞间质转化
Keywords:
lidamycin cervical cancer Hela cells apoptosis autophagy epithelial mesenchymal transformation
分类号:
R329.24; R329.25; R329.28; R73-37; R737.330.22; R979.14
DOI:
10.3969/j.issn.1001-1978.2019.10.015
文献标志码:
A
摘要:
目的 研究力达霉素(LDM)对Hela细胞的细胞周期、凋亡、自噬和上皮细胞间质转化(EMT)的影响及其分子机制。方法 MTT法分析LDM对Hela细胞增殖的影响; 流式细胞术检测LDM对Hela细胞的细胞周期、细胞凋亡的影响; Western blot检测细胞内相关蛋白的表达水平; Transwell法分析LDM对Hela细胞迁移、侵袭能力的影响; QT-PCR分析LDM对Hela细胞赖氨酸特异性去甲基化酶1(LSD1)表达的影响。结果 LDM明显抑制Hela细胞增殖,其不同处理时间的IC50值分别为19.24 ng·mL-1(24 h)、6.678 ng·mL-1(48 h)和3.221 ng·mL-1(72 h)。 LDM可诱导Hela细胞发生G2/M期阻滞和细胞凋亡。低浓度LDM(5 ng·mL-1)可诱导Hela细胞发生自噬,但高浓度LDM(20 ng·mL-1)则抑制Hela细胞的自噬水平。LDM抑制Hela细胞迁移和侵袭,改变EMT相关因子的蛋白表达水平,且这一过程与LSD1无关。结论 LDM能抑制宫颈癌Hela细胞增殖和侵袭转移,其作用机制可能与阻遏细胞周期、诱导细胞凋亡、干扰细胞自噬和抑制EMT密切相关。
Abstract:
Aim To investigate the effects of lidamycin(LDM)on cell cycle, apoptosis, autophagy and epithelial mesenchymal transformation(EMT)of human cervical cancer Hela cells as well as its underlying mechanism.Methods MTT assay was used to analyze the effect of LDM on Hela cell growth.Flow cytometry was used to detect the effect of LDM on cell cycle, apoptosis and mitochondrial membrane potential.Western blot was used to detect the expression levels of proteins.Transwell assay was used to analyze the effect of LDM on migration and invasion of Hela cells.QT-PCR was employed to analyze the effects of LDM on LSD1(lysine specific demethylase 1)expression level in Hela cells.Results LDM could significantly inhibit Hela cell proliferation in a time-and concentration-dependent manner, with the IC50 values of 19.24 ng·mL-1(24 h), 6.678 ng·mL-1(48 h)and 3.221 ng·mL-1(72 h).LDM could induce G2/M cycle arrest and mitochondrial-mediated endogenous apoptosis in Hela cells.Low concentration of LDM(5 ng·mL-1)could induce autophagy, but high concentration of LDM(20 ng·mL-1)could inhibit autophagy in Hela cells.In addition, LDM could inhibit the migration and invasion of Hela cells, change the expression levels of EMT-related factors, but LSD1 was not involved in this process.Conclusions LDM can effectively inhibit proliferation, invasion and metastasis of Hela cervical cancer cells, and its mechanism may be closely related to cell cycle arrest, apoptosis, interfering autophagy and inhibition of EMT.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2019-06-19,修回日期:2019-08-25 基金项目:国家自然科学基金资助项目(No 81772833); 湖北省科技知识创新专项基金资助项目(No 2017CFB556) 作者简介:徐 珊(1989-),女,硕士生,研究方向:基础免疫学,E-mail:676842701@qq.com; 曹春雨(1976-),男,博士,副教授,硕士生导师,研究方向:肿瘤表观遗传学,通讯作者,Tel:0717-6397179, E-mail:407788923@qq.com
更新日期/Last Update: 2019-09-15