[1]刘 杨,段学清,严福林,等.异绿原酸A抑制NLRP3炎性复合体/NF-κB活化减轻胶原诱导型关节炎大鼠炎症反应[J].中国药理学通报,2019,(10):1415-1419.[doi:10.3969/j.issn.1001-1978.2019.10.016]
 LIU Yang,DUAN Xue-qing,YAN Fu-lin,et al.Isochlorogenic acid A inhibits activation of NLRP3/NF-κB in rats with collagen-induced arthritis[J].Chinese Pharmacological Bulletin,2019,(10):1415-1419.[doi:10.3969/j.issn.1001-1978.2019.10.016]
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异绿原酸A抑制NLRP3炎性复合体/NF-κB活化减轻胶原诱导型关节炎大鼠炎症反应()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2019年10期
页码:
1415-1419
栏目:
论著
出版日期:
2019-09-15

文章信息/Info

Title:
Isochlorogenic acid A inhibits activation of NLRP3/NF-κB in rats with collagen-induced arthritis
文章编号:
1001-1978(2019)10-1415-05
作者:
刘 杨1段学清1严福林2潘 春1吴继春2曹 峰1梁 江3
贵州中医药大学 1.基础医学院、2.药学院、3.第一附属医院风湿免疫科,贵州 贵阳 550025
Author(s):
LIU Yang1 DUAN Xue-qing1 YAN Fu-lin2 PAN Chun1 WU Ji-chun2 CAO Feng1 LIANG Jiang3
1.Basic Medical School; 2.College of Pharmacy; 3.Rheumatology and Immunology Dept, the First Affiliated Hospital, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China
关键词:
异绿原酸A 类风湿关节炎 炎症 滑膜病变 骨关节炎 骨破坏
Keywords:
isochlorogenic acid A rheumatoid arthritis inflammation synovial lesion osteoarthritis bone destruction
分类号:
R-332; R284.1; R322.71; R364.5; R593.22; R684.3; R971.1
DOI:
10.3969/j.issn.1001-1978.2019.10.016
文献标志码:
A
摘要:
目的 分析异绿原酸A对胶原诱导型关节炎大鼠抗炎效应与潜在作用机制。方法 SD♂大鼠分为正常组、模型组、异绿原酸A高、低剂量组。高、低剂量异绿原酸A连续给药6周,测量大鼠足跖肿胀度,脾脏脏器系数; HE染色观察骨关节病理改变; Western blot检测关节滑膜NLRP3、caspase-1、NF-κB p65、p-NF-κB p65、p-IκB-ɑ、RANKL蛋白表达; ELISA检测血浆IL-1β、IL-6、TNF-ɑ、CRP、IFN-γ、IL-18表达。结果 与正常组比较,模型组大鼠足跖明显肿胀,脾脏系数变大; 关节滑膜内NLRP3、caspase-1、NF-κB p65、p-NF-κB p65、p-IκB、RANKL蛋白表达明显升高; 血浆IL-1β、IL-6、TNF-ɑ、CRP、IFN-γ、IL-18明显上升。不同剂量异绿原酸A能明显降低模型组上述各项指标。结论 异绿原酸A对胶原诱导型关节炎有较好的抗炎作用,其抗炎活性可能与降低NLRP3炎性复合体激活和NF-κB磷酸化表达有关。
Abstract:
Aim To analyse the anti-inflammatory effect of isochlorogenic acid A in rats with collagen-induced arthritis and the potential mechanism.Methods Male SD rats were randomly divided into normal group, model group, isochlorogenic acid A high and low dose groups.The model was constructed in all groups except control group.After continuous administration for six weeks, the plantar thickness and spleen organ coefficient were measured in rats.NLRP3, caspase-1, NF-κB p65, p-NF-κB p65, p-IκB and RANKL protein in synovium of joint were detected by Western blot, and IL-1β, IL-6, TNF-ɑ, CRP, IFN-γ, IL-18 expressions in blood plasma were detected by ELISA kit.Results Compared with normal group, the swelling degree of plantar and the spleen organ coefficient in model group increased significantly.Western blot showed that the expression of NLRP3, caspase-1, NF-κB p65, p-NF-κB p65, p-IκB-ɑ and RANKL protein were up-regulated in synovium of joint tissues in model group.ELISA showed that the expression of blood plasma IL-1β, IL-6, TNF-ɑ, CRP, IFN-γ and IL-18 were significantly up-regulated in model group.Different doses of isochlorogenic acid A can significantly reduce the performance of the above indicators in model group.Conclusions Isochlorogenic acid A has a good anti-inflammatory effect on collagen-induced arthritis, and its anti-inflammatory activity may be related to the decrease of NLRP3 inflammatory complex activation and NF-κB phosphorylation.

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备注/Memo

备注/Memo:
收稿日期:2019-06-17,修回日期:2019-07-28 基金项目:国家自然科学基金资助项目(No 81560817); 贵州省一流学科建设资助项目(No QYNYL[2017]005); 贵州省科技厅资助项目(No 黔科合平台人才[2017]5735号-08) 作者简介:刘 杨(1986-),男,硕士,讲师,研究方向:类风湿关节炎药理学,Tel:0851-8506145,E-mail:www.liuyang2009@aliyun.com; 梁 江(1983-),男,博士,副教授,研究方向:类风湿关节炎药理学,通讯作者,Tel:0851-85284822,E-mail:gzy20160907@126.com
更新日期/Last Update: 2019-09-15