[1]王妍妍,张 丽,黄春霞,等.七氟醚后处理对失血性休克复苏大鼠海马氧化应激及SIRT1/PGC-1α表达的影响[J].中国药理学通报,2019,(10):1443-1447.[doi:10.3969/j.issn.1001-1978.2019.10.020]
 WANG Yan-yan,ZHAGN Li,HUANG Chun-xia,et al.Effects of sevoflurane postconditioning on oxidative stress and expression of SIRT1/PGC-1α of hippocampus in a rat modelof hemorrhagic shock and resuscitation[J].Chinese Pharmacological Bulletin,2019,(10):1443-1447.[doi:10.3969/j.issn.1001-1978.2019.10.020]
点击复制

七氟醚后处理对失血性休克复苏大鼠海马氧化应激及SIRT1/PGC-1α表达的影响()
分享到:

《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2019年10期
页码:
1443-1447
栏目:
论著
出版日期:
2019-09-15

文章信息/Info

Title:
Effects of sevoflurane postconditioning on oxidative stress and expression of SIRT1/PGC-1α of hippocampus in a rat model of hemorrhagic shock and resuscitation
文章编号:
1001-1978(2019)10-1443-05
作者:
王妍妍张 丽黄春霞朱守峰杨青青胡宪文
安徽医科大学第二附属医院麻醉与围术期医学科,安徽 合肥 230601
Author(s):
WANG Yan-yanZHAGN LiHUANG Chun-xiaZHU Shou-fengYANG Qing-qingHU Xian-wen
Dept of Anesthesiology and Perioperative Medicine,the Second Affiliated Hospital of Anhui Medical University,Hefei 230601,China
关键词:
失血性休克复苏 七氟醚 氧化应激 SIRT1 PGC-1α 脑保护
Keywords:
hemorrhagic shock and resuscitation sevoflurane oxidative stress SIRT1 PGC-1α brain protection
分类号:
R-332; R322.81; R349.1; R605.971; R654.1; R977.6
DOI:
10.3969/j.issn.1001-1978.2019.10.020
文献标志码:
A
摘要:
目的 探讨七氟醚后处理对失血性休克复苏大鼠海马氧化应激,以及对沉默信息调节因子1(silent information regulation 1,SIRT1)和过氧化物酶体增殖物激活受体γ共激活因子1α(peroxisome proliferator-activated receptor γ coactivator-1α,PGC-1α)表达的影响。方法 ♂SD大鼠随机分为假手术组(Sham 组)、失血性休克复苏组(Shock组)、七氟醚后处理组(Sevo组)。Sevo组经失血性休克后,于血液回输即刻吸入2.4%七氟醚,Sham组和Shock组在相应时间点吸入95%O2,5% CO2混合气体,记录放血即刻(T0)、放血结束即刻(T1)、血液回输即刻(T2)和血液回输结束即刻(T3)的平均动脉压(MAP),并采集动脉血进行血气分析。血液回输结束24 h后,检测海马组织丙二醛(MDA)含量和线粒体超氧化物歧化酶(SOD)活性,Western blot测定海马组织中SIRT1和PGC-1α蛋白的表达。结果 与Sham组相比,Shock组MDA含量增加,SOD活性降低,SIRT1和PGC-1α蛋白表达上调(P<0.05); 与Shock组相比,Sevo组MDA含量减少,SOD活性增强,SIRT1和PGC-1α蛋白表达上调(P<0.05)。结论 七氟醚后处理可减轻失血性休克复苏大鼠海马氧化应激,其机制可能与上调SIRT1和PGC-1α的蛋白表达水平有关。
Abstract:
Aim To investigate the effects of sevoflurane postconditioning on oxidative stress and the expression of silent information regulation(SIRT1)and peroxisome proliferator-activated receptor γ coactivator 1α(PGC-1α)in hippocampus of rats subjected to hemorrhagic shock and resuscitation.Methods Male SD rats were randomly divided into sham surgery group(Sham group),shock and resuscitation group(Shock group)and 2.4% sevoflurane postconditioning group(Sevo group).The rats in Sevo group were inhaled 2.4% sevoflurane when received resuscitation after hemorrhagic shock,while rats in Sham and Shock group were treated with 95% O2 and 5% CO2 in the corresponding period.MAP and arterial blood gases were measured at T0(start bleeding),T1(30 min after bleeding),T2(start resuscitation),and T3(30 min after resuscitation).After 24h of surgery,rats with successful model were chosen for the detection of various indexes.The content of malonaldehyde(MDA)in hippocampus and the activity of superoxide dismutase(SOD)in mitochondria isolated from hippocampal tissue were detected.Western blot was used to analyze the protein relative expression levels of SIRT1 and PGC-1α in hippocampus.Results Compared with Sham group,the content of MDA increased,the activity of SOD decreased,and the expression of SIRT1 and PGC-1α increased in Shock group(P<0.05).Compared with Shock group,the content of MDA decreased,the activity of SOD increased,and the expression of SIRT1 and PGC-1α increased in Sevo group(P<0.05).Conclusions Sevoflurane postconditioning can alleviate oxidative stress in hippocampus of a model rat of hemorrhagic shock and resuscitation,which may be correlated with the up-regulation of the protein relative expression levels of SIRT1 and PGC-1α.

参考文献/References:

[1] Liu P,Zhao H,Wang R,et al.MicroRNA-424 protects against focal cerebral ischemia and reperfusion injury in mice by suppressing oxidative stress[J].Stroke,2015,46(2):513-9.
[2] Hu X,Wang J,Zhang Q,et al.Postconditioning with sevoflurane ameliorates spatial learning and memory deficit after hemorrhage shock and resuscitation in rats[J].J Surg Res,2016,206(2):307-15.
[3] Zhang T,Chi Y,Ren Y,et al.Resveratrol reduces oxidative stress and apoptosis in podocytes via Sir2-related enzymes,Sirtuins1(SIRT1)/peroxisome proliferator-activated receptor gamma co-activator 1alpha(PGC-1alpha)axis[J].Med Sci Monit,2019,25:1220-31.
[4] Hu X,Wang J,Zhang L,et al.Postconditioning with sevoflurane ameliorates spatial learning and memory deficit via attenuating endoplasmic reticulum stress induced neuron apoptosis in a rat model of hemorrhage shock and resuscitation[J].Brain Res,2018,1696:49-55.
[5] Dang D D,Saiyin H,Yu Q,et al.Effects of sevoflurane preconditioning on microglia/macrophage dynamics and phagocytosis profile against cerebral ischemia in rats[J].CNS Neurosci Ther,2018,24(6):564-71.
[6] 黄 丽,胡宪文,张慕春.线粒体通透性转换孔在七氟醚后处理减轻失血性休克复苏大鼠脑损伤中的作用[J].中华麻醉学杂志,2018,38(4):413-16.
[6] Huang L,Hu X W,Zhang M C,et al.Role of mitochondrial permeability transition pore in reduction of brain injury by sevoflurane postconditioning in a rat model of hemorrhagic shock and resuscitation[J].Chin J Anesthesiol,2018,38(4):413-16.
[7] Wang J,Wang A,He H,et al.Trametenolic acid B protects against cerebral ischemia and reperfusion injury through modulation of microRNA-10a and PI3K/Akt/mTOR signaling pathways[J].Biomed Pharmacother,2019,112:108692.
[8] Zhao X Y,Lu M H,Yuan D J,et al.Mitochondrial dysfunction in neural injury[J].Front Neurosci,2019,13:30.
[9] 何中洪,郑 茜,魏 静,等.反式-茴香脑对叠氮钠诱导的PC12细胞损伤的保护作用[J].中国药理学通报,2019,35(3):321-6.
[9] He Z H,Zheng Q,Wei J,et al.Protective effect of trans-anethole on PC12 cell injury induced by sodium azide[J].Chin Pharmacol Bull,2019,35(3):321-6.
[10] 田崇梅,夏道宗,邢梦雨,等.亚硒酸钠通过Keap1/Nrf2/ARE信号通路诱导人肺腺癌A549细胞凋亡[J].中国药理学通报,2019,35(2):181-6.
[10] Tian C M,Xia D Z,Xing M Y,et al.Sodium selenite induced human lung cancer A549 cells apoptosis through Keap1/Nrf2/ARE signaling pathway[J].Chin Pharmacol Bull,2019,35(2):181-6.
[11] Zhang J F,Zhang Y L,Wu Y C.The role of Sirt1 in ischemic stroke:pathogenesis and therapeutic strategies[J].Front Neurosci,2018,12:833.
[12] Fu B,Zhang J,Zhang X,et al.Alpha-lipoic acid upregulates SIRT1-dependent PGC-1alpha expression and protects mouse brain against focal ischemia[J].Neuroscience,2014,281:251-7.
[13] Tian L,Cao W,Yue R,et al.Pretreatment with Tilianin improves mitochondrial energy metabolism and oxidative stress in rats with myocardial ischemia/reperfusion injury via AMPK/SIRT1/PGC-1 alpha signaling pathway[J].J Pharmacol Sci,2019,139(4):352-60.
[14] Lu X,Zhang L,Li P,et al.The protective effects of compatibility of Aconiti Lateralis Radix Praeparata and Zingiberis Rhizoma on rats with heart failure by enhancing mitochondrial biogenesis via Sirt1/PGC-1alpha pathway[J].Biomed Pharmacother,2017,92:651-60.

备注/Memo

备注/Memo:
收稿日期:2019-07-20,修回日期:2019-08-11 基金项目:国家自然科学基金资助项目(No 81471341) 作者简介:王妍妍(1994-),女,硕士生,研究方向:麻醉药与脑保护,E-mail:1404723408@qq.com; 胡宪文(1972-),男,博士,副教授,博士生导师,研究方向:麻醉药与脑保护,通讯作者,E-mail:huxianwen001@126.com
更新日期/Last Update: 2019-09-15