[1]刁婷婷,张雨晨,吕 伟,等.山楂叶总黄酮对胶质瘤U87细胞的抑制作用[J].中国药理学通报,2019,(10):1448-1452.[doi:10.3969/j.issn.1001-1978.2019.10.021]
 DIAO Ting-ting,ZHANG Yu-chen,LYU Wei,et al.Experimental study on inhibitory effect of hawthorn leaves flavonoids on human glioblastoma U87 cells[J].Chinese Pharmacological Bulletin,2019,(10):1448-1452.[doi:10.3969/j.issn.1001-1978.2019.10.021]
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山楂叶总黄酮对胶质瘤U87细胞的抑制作用()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2019年10期
页码:
1448-1452
栏目:
论著
出版日期:
2019-09-15

文章信息/Info

Title:
Experimental study on inhibitory effect of hawthorn leaves flavonoids on human glioblastoma U87 cells
文章编号:
1001-1978(2019)10-1448-05
作者:
刁婷婷12张雨晨1吕 伟1闵 清1
1.湖北科技学院药学院,湖北 咸宁 437100; 2.湖北省咸宁市中心医院药学部,湖北 咸宁 437100
Author(s):
DIAO Ting-ting12ZHANG Yu-chen1LYU Wei1MIN Qing1
1.School of Pharmacy, Hubei University of Science and Technology, Xianning Hubei 437100,China; 2.Department of pharmacy, The central Hospital of Xianning, Xianning Hubei 437100,China
关键词:
山楂叶总黄酮 人胶质母细胞瘤U87细胞 增殖 迁移 侵袭 黏附
Keywords:
hawthorn leaves flavonoids human glioblastoma U87 cell proliferation migration invasion adhesion
分类号:
R284.1; R329.24; R730.264; R739.410.22
DOI:
10.3969/j.issn.1001-1978.2019.10.021
文献标志码:
A
摘要:
目的 研究山楂叶总黄酮对胶质瘤U87细胞的抑制作用。方法 体外培养U87细胞,采用25、50、100 mg·L-1的山楂叶总黄酮,通过CCK-8实验、划痕实验、Transwell法、黏附实验,检测山楂叶总黄酮对U87细胞增殖、迁移能力、侵袭能力、黏附能力的影响; 克隆形成实验检测细胞克隆形成能力。结果 与空白对照组相比,山楂叶总黄酮呈浓度依赖性抑制U87细胞的增殖、迁移、侵袭及黏附能力,差异具有统计学意义(P<0.05),山楂叶总黄酮50 mg·L-1时抑制胶质瘤U87细胞生长作用最为明显。结论 山楂叶总黄酮对胶质瘤U87细胞有一定的抑制作用。
Abstract:
Aim To explore the inhibitory effect of hawthorn leaves flavonoids(HLF)on human glioblastoma U87 cells.Methods U87 cells were cultured in vitro.The concentration of HLF was 25, 50, 100 mg·L-1 as drug concentration.The effects of HLF on the proliferation, migration, invasion and adhesion of U87 cells were detected by CCK-8 assay, scratch assay, Transwell assay and adhesion assay, and clone forming ability was detected by cell clonogenic assay.Results HLF inhibited the proliferation, migration, invasion and adhesion of U87 cells in a concentration-dependent manner, and the difference was statistically significant compared with control group(P<0.05).When HLF reached 50 mg·L-1, the inhibition of tumor growth was the most significant.Conclusion HLF has a certain inhibitory effect on glioma U87 cells.

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相似文献/References:

[1]闵 清,白育庭,刘 晶,等.山楂叶总黄酮对大鼠血管环的作用及其机制探讨[J].中国药理学通报,2011,(04):585.
 MIN Qing,BAIYu-ting,LIU Jing,et al.Effects and m echanism s of hawthorn leaf fla-vonoids on isolated rat thoracic aorta [J].Chinese Pharmacological Bulletin,2011,(10):585.

备注/Memo

备注/Memo:
收稿日期:2019-06-18,修回日期:2019-07-12 基金项目:湖北省卫生厅中医药研究项目(No 2012Z-B02); 咸宁市科技局科研项目(No 201101) 作者简介:刁婷婷(1992-),女,硕士生,研究方向:心血管药理学和中药药理学,Tel:0715-8130584, E-mail:156793000@qq.com; 闵 清(1967-),女,博士,教授,硕士生导师,研究方向:心血管药理学和中药药理学,通讯作者,Tel:0715-8130584, E-mail:baimin0628@163.com
更新日期/Last Update: 2019-09-15