[1]吴 鹏,魏 盼,李恋曲,等.桂枝汤通过抑制淋巴细胞向Th2细胞分化抑制变应性接触性皮炎[J].中国药理学通报,2019,(10):1461-1466.[doi:10.3969/j.issn.1001-1978.2019.10.023]
 WU Peng,WEI Pan,LI Lian-qu,et al.Guizhi decoction suppresses mouse allergic contact dermatitis by inhibiting differentiation of lymphocytes to Th2 cells[J].Chinese Pharmacological Bulletin,2019,(10):1461-1466.[doi:10.3969/j.issn.1001-1978.2019.10.023]
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桂枝汤通过抑制淋巴细胞向Th2细胞分化抑制变应性接触性皮炎()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2019年10期
页码:
1461-1466
栏目:
复方药物药理学
出版日期:
2019-09-15

文章信息/Info

Title:
Guizhi decoction suppresses mouse allergic contact dermatitis by inhibiting differentiation of lymphocytes to Th2 cells
文章编号:
1001-1978(2019)10-1461-06
作者:
吴 鹏魏 盼李恋曲王晓钰贾志荣洪 敏
南京中医药大学,江苏省中药药效与安全性评价重点实验室,江苏 南京 210046
Author(s):
WU Peng WEI Pan LI Lian-qu WANG Xiao-yu JIA Zhi-rong HONG Min
Nanjing University of Chinese Medicine, Jiangsu Key Lab for Pharmacology and Safety Evaluation of Chinese Materia Medica, Nanjing 210046,China
关键词:
桂枝汤 变应性接触性皮炎 FITC 淋巴细胞 1型辅助T细胞 2型辅助T细胞
Keywords:
Guizhi decoction allergic contact dermatitis FITC lymphocytes type 1 T helper cells type 2 T helper cells
分类号:
R-332; R289.5; R331.125; R392.12; R758.2
DOI:
10.3969/j.issn.1001-1978.2019.10.023
文献标志码:
A
摘要:
目的 利用异硫氰酸荧光素(FITC)诱导的小鼠变应性接触性皮炎模型以及小鼠脾淋巴细胞,探讨桂枝汤对变应性接触性皮炎的治疗作用和机制。方法 BALB/c小鼠腹部刮毛后,在d 1、2用质量分数为1.5% FITC溶液涂抹腹部致敏,d 6用质量分数为0.6% FITC溶液涂抹小鼠右耳进行激发,d 1~7灌胃给予桂枝汤。激发24 h后测定小鼠左右耳厚,打耳圆片; 检查耳组织的病理。测定耳匀浆中IL-4、IL-5、IL-9、IFNγ,以及血清中IgE的水平。利用ConA刺激小鼠脾淋巴细胞,检测IL-4、IL-10、GATA3以及IFNγ、T-bet的表达水平。结果 桂枝汤明显抑制小鼠耳肿胀度,缓解耳组织中炎性细胞浸润和水肿; 降低耳匀浆中IL-4、IL-9、IFNγ以及血清中IgE的水平; 抑制ConA刺激下脾淋巴细胞中IL-4、IL-10、GATA3及IFNγ的表达。结论 桂枝汤能明显抑制FITC诱导的小鼠变应性接触性皮炎,其机制可能与直接抑制淋巴细胞向Th2细胞分化有关。
Abstract:
Aim To reveal the effects of Guizhi decoction(GZD)on allergic contact dermatitis by establishing the mouse allergic contact dermatitis model induced by fluoresciniso thiocyate(FITC)and culturing splenic lymphocytes.Methods BALB/c mice were sensitized by smearing the 1.5% FITC on the shaved abdomens on day 1 and day 2 and elicited on the right ear with 0.6% FITC solution on day 6.GZD was administered from day 1 to day 7.Ear thickness was measured 24 h after elicitation.HE staining was performed for pathohistological examination.Interleukin-4(IL-4), IL-5, IL-9, interferon γ(IFNγ)in ear tissue homogenates and IgE in serum were detected.The splenic lymphocytes were stimulated with ConA.Results The ear swelling was significantly suppressed by administering GZD.GZD reduced the infiltration of inflammatory cells and edema in the ear, and the levels of IL-4, IL-9, IFNγ and IgE.GZD could also reduce the expression of IL-4, IL-10, IFNγ and GATA3.Conclusions GZD could suppress allergic contact dermatitis elicited by FITC, which may result from inhibiting the differentiation of lymphocytes to Th2 cells.

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备注/Memo

备注/Memo:
收稿日期:2019-07-10,修回日期:2019-08-12 基金项目:国家自然科学基金面上项目(No 81473395) 作者简介:吴 鹏(1992-),男,硕士生,研究方向:中药药理学,E-mail:1083175265@qq.com; 洪 敏(1972-),女,博士,教授,博士生导师,研究方向:中药免疫药理学,通讯作者,E-mail:hongmin72@126.com
更新日期/Last Update: 2019-09-15