[1]刘春燕,杨胜辉,朱 伟,等.金匮肾气汤对糖尿病肾病小鼠足细胞凋亡信号的影响[J].中国药理学通报,2019,(10):1466-1470.[doi:10.3969/j.issn.1001-1978.2019.10.024]
 LIU Chun-yan,YANG Sheng-hui,ZHU Wei,et al.Effect of Jinguishenqi decoction on apoptosis signal of podocyte in diabetic nephropathy mice[J].Chinese Pharmacological Bulletin,2019,(10):1466-1470.[doi:10.3969/j.issn.1001-1978.2019.10.024]
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金匮肾气汤对糖尿病肾病小鼠足细胞凋亡信号的影响()
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《中国药理学通报》[ISSN:/CN:]

卷:
期数:
2019年10期
页码:
1466-1470
栏目:
复方药物药理学
出版日期:
2019-09-15

文章信息/Info

Title:
Effect of Jinguishenqi decoction on apoptosis signal of podocyte in diabetic nephropathy mice
文章编号:
1001-1978(2019)10-1466-05
作者:
刘春燕1杨胜辉2朱 伟1曾 辉3魏 科4唐 群1
湖南中医药大学1.病理学教研室、2.预防医学教研室、3.生理学教研室、4.病原生物学教研室,湖南长沙 410208
Author(s):
LIU Chun-yan1 YANG Sheng-hui2 ZHU Wei1 ZENG Hui3 WEI Ke4 TANG Qun1
1.Dept of Pathology; 2.Dept of Preventive Medicine; 3.Dept of Physiology; 4.Dept of Microbiology, Hunan University of Chinese Medicine, Changsha 410208, China
关键词:
金匮肾气汤 凋亡 糖尿病肾病 足细胞 JNK1 Bcl-2
Keywords:
Jinguishenqi decoction apoptosis diabetic nephropathy podocyte JNK1 Bcl-2
分类号:
R-332; R289.5; R322.61; R329.25; R692.390.531
DOI:
10.3969/j.issn.1001-1978.2019.10.024
文献标志码:
A
摘要:
目的 探讨金匮肾气汤对db/db糖尿病小鼠肾脏足细胞凋亡的影响及机制。方法 将6周龄db/db糖尿病小鼠随机分为生理盐水组、金匮肾气汤组、二甲双胍组,均灌胃给药; ♂ db/m小鼠作为正常对照组。于18周龄处死动物,取血检测血糖,留24 h尿检测尿白蛋白; 留取肾脏组织,分别进行PAS染色观察肾小球内系膜增生情况,透射电镜观察肾小球内足细胞足突情况,免疫荧光检测podocin在肾组织的表达,Western blot检测caspase-3、p-Bcl-2、p-JNK1/JNK1在肾组织的表达。结果 与db/db糖尿病小鼠比较,金匮肾气汤治疗后,24 h尿白蛋白水平降低,肾小球内系膜增宽程度减轻,肾小球内足细胞足突倒伏、融合明显减轻,肾小球内podocin线样荧光明显增强,肾皮质内caspase-3表达明显降低(P<0.05),JNK1和Bcl-2磷酸化明显下调(P<0.05)。结论 金匮肾气汤通过JNK1/Bcl-2信号途径,改善足细胞凋亡,保护足细胞,延缓糖尿病肾病的进展。
Abstract:
Aim To investigate the effect of Jinguishenqi decoction on renal podocyte apoptosis in db/db diabetic mice and the underlying mechanism.Methods Six weeks old db/db diabetic mice were randomly divided into three groups: saline group, Jinguishenqi decoction group, metformin positive control group, and male db/m mice as normal control group.The animals were sacrificed at 18 weeks old,urinary albumin was detected in 24 hours urine, and blood glucose was measured.The glomerular mesangial proliferation was observed by PAS staining in renal tissues.The foot processes in podocyte were observed by transmission electron microscopy.The expression of podocin in renal tissues was detected by immunofluorescence.The protein expressions of caspase-3, p-Bcl-2, p-JNK1/JNK1 in renal cortex were detected by Western blot.Results Compared with saline group of db/db diabetic mice, urinary albumin in 24 hours urine, foot process fusion and mesangial width were all reduced after the treatment of Jinguishenqi decoction.Podocin fluorescence in glomerular increased, and the expression of caspase-3 in renal cortex decreased significantlyafter the treatment of Jinguishenqi decoction(P<0.05).The phosphorylation of JNK1 and Bcl-2 was inhibitedsignificantly after the treatment of Jinguishenqi decoction(P<0.05).Conclusions Jinguishenqi decoction could alleviate apoptosis of podocyte, protect podocyte and delay the progression of diabetic nephropathy through JNK1/Bcl-2 signaling pathway.

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备注/Memo

备注/Memo:
收稿日期:2019-07-12,修回日期:2019-08-15 基金项目:国家自然科学基金资助项目(No 81603470,81503442) 作者简介:刘春燕(1979-),女,博士,讲师,研究方向:中西医结合防治慢性肾脏病,E-mail: liuchunyan0221@126.com; 唐群(1980-),男,博士,副教授,研究方向:中西医结合防治慢性肾脏病,通讯作者,E-mail: tangqun460@126.com
更新日期/Last Update: 2019-09-15